Studies on production, purification and molecular characterisation of polygalacturonase from Bacillus subtilis.
M.P.Kusuma, M.Vijaya Bhargavi
Polygalacturonases are responsible for the degradation of the long and complex molecules called pectin that occur as structural polysaccharides in the middle lamella and the primary call walls of young plant cells. Pectinases are an integral part of fruit juice and textile industries as well as having various biotechnological applications. Polygalacturonase was produced by SMF using an isolate Bacillus subtilis. The enzyme was purified and the purity was judged by SDS PAGE. A preliminary structural and physico- chemical characterization of polygalacturonase has been performed by N terminal sequencing, accurate molecular mass determination by MALDI-TOF mass spectrometry and secondary structure analysis by circular dichroism.