Morphological, Physiological and Molecular studies on wildly collected Cordyceps militaris from North West Himalayas, India
Pooja Pathania, Madhavi Joshi, Anand Sagar
Cordyceps militaris (L.) Link is an entomopathogenic fungus, which enjoyed an extensive praise for its medicinal functions. In this study, taxonomic details, isolation of pure culture, influence of different physiological requirements on the mycelia growth of this fungus its chemical components and molecular characterization has been carried out. A small bit of tissue from stipe and stroma was used as material for in vitro isolation. The present investigation revealed that this fungus showed optimum growth in YPDA (yeastal potato dextrose agar) and GAS (glucose asparagine solution). The maximum mycelial growth was observed at 25 ºC and pH 7.5 and 5.5 in solid and liquid media respectively. Among various carbon, nitrogen, mineral and vitamin sources tested, sucrose, beef extract, zinc chloride and folic acid produced the maximum mycelial yield respectively. The fungus was found rich in various chemical components like vitamins, proteins trace elements, cordycepin and cordycepic acid. Molecular characterization of fungus resulted in amplified product of 540 basepairs, which is submitted in NCBI database having submission ID KJ004029. C. militaris has been successfully cultivated under lab scale cultivation trials under standardized nutritional and climatic conditions.