Vol. 3, Issue 4 (2015)
Five fungal species were isolated from an abandoned heavy metals contaminated soil, identified as Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Mucor mucedo and Trycophyton mentagrophyte and were screened for biosurfactants production using oil spreading technique and emulsification stability test. Aspergillus niger and Aspergillus flavus produced biosurfactants on the broth of Mineral Salt Medium containing 2% of crude oil. These biosurfactants were partially purified and characterized by Thin Layer Chromatography and Gas Chromatography-Mass Spectrometry analysis. The analyses signify Glycolipid biosurfactants specifically designated as glycosophorolipid Sf1 and glycosophorolipid Sf2 respectively, containing fatty acids with various functional groups.
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