In vitro callus induction and plants from leaf explants of Wattakaka volubilis L. (Staf. Arn)- An vulnerable medicinal plant
Dhanapaul Vinothkumar, Murugesan Senthilkumar
Callus regeneration from leaf explants of Wattakaka volubilis an important medicinal plant inoculated on MS medium supplemented with 0.6mg/l BAP and 0.2mg/l NAA, produced numerous (26.4±0.48). Shootlets with an average length of 9.6±0.04 cm. Small shootlets were transferred to shoot elongation medium supplemented with 0.2mg/l BAP plus 0.4mg/l GA3. Elongated shoots produced roots within 6 weeks in half strength MS basal medium supplemented with 0.6mg/l IBA and 3% (w/v) sucrose (12.2±0.40). Rooted plantlets were acclimatized with a survival rate of 95% and transferred to the green house and thereafter successfully transferred to the field with 90% survival rate.