Vol. 3, Issue 7 (2015)
Evaluation of Genotypic Variation Using SDS-PAGE
Author(s): Pramod Kr. Singh, Neha Panwar, Sameer S. Bhagyawant
Abstract: The objective of this study was to assess the genetic divergence available in four different genotypes ofrnchickpea based on their analysis of seed storage proteins, for the identification of genetically diverse andrnagronomically superior genotypes of chickpea seeds. In this study, total 4 cultivars of chickpea obtainedrnfrom Sehore M.P. have been studied for their analysis of seed storage protein profiles to examine theirrnrelationship by SDS-PAGE technique. The Proteomic assay comprised a total of 64 reliably score ablernprotein alleles identified in the 4 accessions of legumes. There is no polymorphic band found in thisrnresult so these accessions cannot be further differentiated different genotypes of chickpea and not usedrnfurther in breeding programmes.