European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience

ISSN: 2321-9122

Vol. 3, Issue 7 (2015)

Susceptibility pattern of Pseudomonas aeruginosa to aminoglycosides (Gentamicin and Amikacin) in a tertiary care hospital of Karachi, Pakistan

Author(s): Roofia Tanvir, Shumaila Ather, Ali Shariq, Syed Bilal Tanvir, Sumaira Ahmed, Arif Hussain
Abstract: Objective: To deduce the resistance pattern of multidrug resistant P.aeruginosa isolates tornaminoglycosides (Amikacin and Gentamicin) from clinicalspecimens obtained from a tertiary care hospitalrnin Karachi, Pakistan.
Methods: A prospective crossectional study was undertaken from January 2014 to January 2015 at arnprivate tertiary care hospital in Karachi. Collection of specimens was carried out from three differentrnunits across the city. Customary and precise bacteriological procedures were used to detect the clinicalrnisolates. The isolates were cultured on chocolate and MacConkey agar. Utilizing the Kirby Bauer Discrndiffusion method on Mueller-Hinton Agar the sensitivity patterns were deduced.
Results: A total of 1622 isolates of P. aeruginosa were cultured during the study’s time frame spanningrnover a year. Cultures which were found to be positive were then tested against Amikacin and Gentamicinrnand their pattern of susceptibility were ascertained.
Conclusions: MDR P. aeruginosa isolates show an advanced pattern of resistance as compared tornprevious studies. The amplified pattern of resistance of isolates to Amikacin and Gentamicin wasrnobserved.
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