Vol. 3, Issue 7 (2015)
Isolation, characterization and identification of heavy metal tolerating thermophiles from hot water spring
Author(s): Sonali Patil, Geetha Unnikrishnan
Abstract: Many industries such as metal finishing / metal plating, gold and silver refining, petroleum refining, ironrnand steel use lead (Pb), cadmium (Cd), zinc (Zn),mercury (Hg), arsenic (As), silver (Ag) chromiumrn(Cr),copper (Cu) iron (Fe), platinum group elements and other heavy metals as important ingredients torndevelop various types of metal alloys which are the major contributors to water as well as soil pollutionrnas they create more subtle effects on behavior, reproduction or even survival of biotic communities. Anrnenrichment culture technique and molecular biology technique was used to isolate bacterial strains fromrnhot water springs of Vajreshwari and Ganeshpuri which can tolerate high concentrations of the selectedrnheavy metals. Five pure bacterial cultures, named SZP 4, SZP 8, SZP 12, SZP 16, SZP 18 were isolatedrnand subsequently characterized by 16S rRNA gene sequencing and biochemical tests. The optimumrntemperature, pH and NaCl concentration was determined for them. Tolerance to high levels of metal saltsrn(Cd, Cr, Cu, Fe, and Zn) was seen in all five bacterial isolates. By SDS – PAGE analysis it was foundrnthat there is presence of specific proteins indicating that the resistant trait observed was specificallyrnoperon mediated. Thus such findings may be useful in designing a multi resistant bacterium that can bernused to return the altered environment to its original condition.