European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience

ISSN: 2321-9122

Vol. 3, Issue 7 (2015)

Production of New Thermostable and Acidstable Alpha-Amylase from Bacillus sp.B1 and B2 under Solid State Fermentation

Author(s): Sorour Sharifi Alghabpoor, Armen Trchunian, Mohammad Hosseinpour
Abstract: This study was conducted to identify and screen native Bacillus strains from hot spring samples for alpharnamylase activity. Positive strains for the enzyme activities were Bacillus, SP.B1 and Bacillus, SP.B2.Thernisolated cultures screened by solid state fermentation using wheat bran as its substrate. A crude extractrnenzyme applied to amylase assay to determine the enzyme’s activity against standard curve of maltose.rnAs a result, wheat bran was shown to be the normal solid organic substrate for the highest alpha amylasernproduction with 244.10 and 187.15 U/ml/min with pH 5 and 5.5in Bacillus.Sp.B2 and Bacillus.Sp.B1,rnrespectively in 48 h. The incubation temperature 50°C was found to be optimum for the production ofrnalpha amylase. The enzymes were stable for 30 min between 65°C-70°C
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