European Journal of Biotechnology and Bioscience

ISSN: 2321-9122

Vol. 3, Issue 9 (2015)

Investigation of acute toxicity and the effect of chrome plating industry effluent on behavior of the guppy (Poecilia reticulata)

Author(s): J. Ahila Angelin, R. D. Stevens Jones, S. Sam Manohar Das
Abstract: In this study 96-h LC50 value of chromium, is a metal widely used in industry, was determined for thernguppy (Poecilia reticulata, Peters, 1859). 10 fish were placed in each replicate of each dose. Thernexperiments were performed as three replicates, and behavioural changes in the guppy were determinedrnfor each chrome plating effluent concentration. The data obtained were statistically evaluated by thernuse of EPA computer program based on Finney’s Probit Analysis Method and a 96-h LC50 value for P.rnreticulata was found to be 1.67 ml/l in a static bioassay test system. Lower and upper confidence limitsrnfor the LC50 were 1.44 and 1.94 ml/l, respectively. The behavioural changes observed in fish werernswimming in imbalanced manner, capsizing, attaching to the surface, difficulty in breathing andrngathering around the ventilation filter
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