Vol. 4, Issue 12 (2016)
Study of Auxin, cytokinin and gibberellin-like activity of heterocyclic compounds derivatives of pyrimidine, pyridine, pyrazole and isoflavones
Author(s): Victoria Tsygankova, Yaroslav Andrusevich, Olexandra Shtompel, Olexandr Myroljubov, Artem Hurenko, Roman Solomyanny, Galyna Mrug, Mykhaylo Frasinyuk, Oleg Shablykin, Volodymyr Brovarets
Abstract: The phytohormone-like activity of synthetic low molecular weight heterocyclic compounds derivatives of pyrimidine, pyridine, pyrazole, and isoflavones was studied. Testing of stimulating effect of synthetic heterocyclic compounds on seed germination and seedling growth of flax (Linum usitatissimum L.) of cultivar Ukrainian 3 showed expressive auxin-like activity of these compounds. The growth parameters of 15th-day-old flax seedlings grown on the solution of heterocyclic compounds used at concentration 10-8M/l of distilled water were higher or similar to growth parameters of flax seedlings grown on the water solution of auxins IAA and NAA used at the same concentration as compared to lower growth parameters of control flax seedlings grown on the distilled water (control). The specific bioassay on auxin-like activity showed high stimulating effect of heterocyclic compounds derivatives of pyrimidine, pyridine, pyrazole, and isoflavones used at concentration 10-8M/l of distilled water on rhizogenesis on the 14th-day-old leaf petioles isolated from seedlings of haricot bean (Phaseolus vulgaris L.) of cultivar Belozernaya. The specific bioassay on cytokinin-like activity conducted on the 16th-day-old cotyledons isolated from seeds of muscat pumpkin (Cucurbita moschata Duch. et Poir.) of cultivar Gilea, showed that heterocyclic compounds derivatives of pyrimidine, pyridine, pyrazole, and isoflavones used at concentration 10-8M/l of distilled water demonstrated high stimulating activity on growth of biomass of isolated cotyledons of pumpkin, which was similar or higher than the activity of cytokinin Kinetin. The specific bioassay on gibberellin-like activity conducted on the 3rd- day-old seedlings of lettuce (LactucasativaL.) of cultivar Berlin testified that some heterocyclic compounds derivatives of pyrazole, isoflavones, and pyridine used at concentrations ranging from 10-4M to 10-9M/1 of distilled water revealed minor stimulating effect on elongation of hypocotyl of lettuce seedlings in relation to control, but their activity was significantly lower than activity of gibberellic acid GA3. This bioassay showed also that the some heterocyclic compounds derivatives of pyrazole and isoflavones used at concentration 10-7M/l of distilled water demonstrated nonspecific for this bioassay auxin-like activity, which manifested in stimulation of formation and growth of root system on the seedlings of lettuce. Obtained results confirmed specific auxin-like and cytokinin-like, and minor gibberellin-like effect of synthetic heterocyclic compounds on cell division, cell proliferation, cell elongation and cell differentiation that are the basic processes of plant growth and development.