The effect of long-term exposure to low frequency electromagnetic field on cholinergic system and nitric oxide level of isolated distal colon of male rats
Mina Adampourezare, Aminallah Bahaodini, Leila Mehdizadeh Fanid
Objective: To evaluate the effects of long-term exposure to low frequency electromagnetic field(EMF) on the cholinergic system and nitric oxide level of isolated distal colon.
Methods: Fourteen adult male Sprague-Dawley rats were randomly divided into sham and experimental groups. Experimental group was exposed to 1mT and 50Hz electromagnetic field, continuously for 140 days 24 hrs per day in a solenoid. Sham group was housed under conditions same as experimental group, without EMF. At the end of the exposure period, the animals were anaesthetized with ether and the distal colon was removed and the tension changes of distal colon tissue were recorded in response to acetylcholine and L-NG-nitroarginine methyl ester (L-NAME).
Results: The tension changes of distal colon tissue to acetylcholine and L-NAME was higher in the experimental group in compared to the sham group. Significant difference was detected between groups (p<0.05).
Conclusion: Long-term exposure to low frequency electromagnetic field increased sensitivity of acetylcholine receptors and decreased produce of nitric oxide in distal colon.