RFLP assessment for genetic diversity among Indian Poultry birds of Himalayan Region
Ahmed Kareem Alatafi, kondapalli kasturi
As a result of many years of domestication and breeding, a wide variety of chicken breeds exist today. However, because of many malpractices an increasing number of local breeds are under threat of extinction rendering many valuable genotypes and traits at risk of being lost. The genetic erosion of these local breeds may lead to the loss of valuable genetic variability in specific characteristics that are momentarily unimportant in commercial breeding strategies. The present work deals with applying the RFLP Technique on a selected gene (IGF1) to look for the genetic diversity within 10 individuals of Red Fowl and Black Fowl of Himalayan Region. The work is further extended on bioinformatics level wherein the sequence obtained for one of the randomly selected amplicon was locally aligned to search its similarity with the closest sequence(s) for tracing the phylogeny.