Effect of sterilizing agents on the production of callus in medicinal plant: Chlorophytum sps.
Jitendriya Panigrahi, Illa C Patel
Chlorophytum borivilianum Santapau & R.R. Fern and Chlorophytum tuberosum (Roxb.)rnBaker has been considered as critically endangered plants belongs to familyrnAnthericaceae. Different sterilants with different combinations of Dettol,rnantioxidant solution (ascorbic acid+ citric acid+ autoclaved distilled water),rnethanol, hydrogen peroxide, and sodium hypochlorite were used. Among themrnleaves treated with hydrogen peroxide showed significant growth and survivalrnrate as compared to sodium hypochlorite and antioxidant solutions and dettolrntreated leaves. The most effective protocol for sterilization tested was washedrnwith distilled water followed by 3% hydrogen peroxide for 5minutes and thenrnrinsed with ethanol. For callus production, leaves were taken as explant andrngrown under full strength Murashige and Skoog media+ cytokinin (Benzylrnadenine-BA) either alone or with 2,4-Dichlorophenoxy acetic acid (2,4-D) atrndifferent concentrations on callus. The best results shown was BA 0.7 µm+2, 4 Drn0.7µm with 100% callus production. This protocol could be used for secondaryrnmetabolite production along with micropropagation of these endangeredrnvarieties.