In spite of few successful reports ofrnblackgram (Vigna mungo L. Hepper) regeneration, their survival rate isrnlow on transplantation as these in vitro raised plantlets are usuallyrnsensitive to hardening as well as transplantation to soil. The survival raternduring hardening and transplantation has been reported to be 65-70%. This studyrnevaluated the response of tissue culture raised blackgram to arbuscularrnmycorrhizal (AM) fungi, Fasciculatum and to the nitrogen fixing bacteriarnRhizobium leguminosarum, recovers during hardening process andrntransplantation shock, growth, nodulation and nitrogen fixation activity. Thernoverall survival rate of plants increased up to 90-95% in the in vitrornraised tissue cultured plants. The results also demonstrated that the dualrninoculation of in vitro grown blackgram plants with Rhizobium and AMrnfungi significantly increased the percentage of root colonization, plantrnbiomass, nodulation, and nitrogen fixation activity in comparison with thernplantlets inoculated with either Rhizobium or AM fungi alone.