Vol. 5, Issue 1 (2017)
In this study we isolated an acetic acid bacterial strain from a home-made wine sample and investigated its cellulose production. The strain was identified using biochemical tests and 16S rRNA gene sequencing and named at the species level as Acetobacter pasteurianus. We investigated cellulose production by Acetobacter pasteurianus under the utilization of various carbon (glucose, sucrose, fructose, and ethanol) and nitrogen (yeast extract, casein hydrolysate, and ammonium sulphate) sources and industrial wastes (molasses, corn steep liquor, whey, and olive mill wastewater). The results showed that the best carbon and nitrogen sources for the production of cellulose were glucose and yeast extract. In addition, the best production occurred when molasses was used as the industrial waste. Bacterial and plant celluloses are reticulated and unbranched polymers of β-1, 4-linked glucopyranose units. Therefore, the structural characteristics of cellulose were examined using Thin-layer chromatography (TLC), Fourier transform infrared spectroscopy (FTIR) and Carbon-13 nuclear magnetic resonance (C13NMR) methods. The structural characteristics of bacterial cellulose were similar to those of plant cellulose. In addition, the porous and reticulated structure of bacterial cellulose was viewed using Scanning electron microscopy (SEM).
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