Production of biofuel from fruits and vegetable waste has been carried out with the singular aim of converting the waste to useful material. The Bioethanol was produced by using fruit waste like Indian water chestnut, sweet potato, jackfruit and pineapple. To achieve this, the conversion of fruits and vegetable wastes were respectively carried out via acid hydrolysis, which yielded fermentable sugar. A pure culture of Saccharomyces cerevisiae was used to carry out the fermentation process. It was seen that after fermentation, pineapple waste produced 0.090% (0.90 mg/ml) ethanol, sweet potato waste produced 0.079% (0.79 mg/ml) ethanol, Indian water chestnut waste produced 0.045% (0.45ml/ml) ethanol whereas jackfruit waste produced 0.045% (0.45 mg/ml) ethanol. The study considered the enormous amount of fruits and vegetable wastes thrown away daily in every household, and therefore embarked upon the conversion of these wastes into a useful product, ethanol.