European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 5, Issue 5 (2017)

Screening of amylase producing microbes from rhizosphere soil and its potential application in baking bread


Queen J, Rajalakshmi G, Komathi S

Improvement of microbial strains for the production of industrial products has been a hallmark of all commercial, microbial fermentation processes. The present investigation deals with the isolation and characterization of microbes for the enhancement of seed germination collected from Coimbatore. Out of 5 isolate were selected by screening test. Based on morphologically, and biochemical characterization the isolates were identified as Pseudomonas sp, Bacillus sp, Ecoli sp, Proteus sp, Shigella sp. The isolated bacterial strains were subjected to mutation process for strain improvement by sequential treatment with physical agents (UV) and chemical agents (EMS). These mutant strains were subjected for the production of lytic enzymes like protease and amylase. Amylase is an enzymes which breaks the starch into sugar, usually important constituent of the human diet. These amylase enzymes are used for the baking industries for baking bread. Further, it was subjected for the application of baking industries for making bread by using amylase enzymes.
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