Screening and identification of bacterial strains from milk industrial waste using 16s rRNA ribotyping
Suresh Kumar, Monika Verma, Ankur R Bhardwaj, Anand Pushkar
PCR based 16S rRNA gene profiling or ribotyping is a widely used method to accurately identify and differentiate bacterial species and strains. In the present study authors have attempted to identify bacterial species growing in waste material of milk industry. For this purpose waste material was collected form the Ballabgarh Co-operative Milk Producer Union plant situated at Ballabgarh, Haryana, India. Bacterial DNA was isolated from purified bacterial culture and subsequently utilized to PCR amplify 16S rRNA region. Sanger’s method of sequencing and other computational methods were employed to screen the homology of amplified PCR products against known bacterial species.