A field experiment was conducted during the year 2016-17 at Instructional farm, College of Agriculture, JAU, Junagadh to study the effect soil and foliar application of zinc and iron in wheat on micronutrients uptake in calcareous soil of Saurashtra region. The soil was clayey in texture, calcareous in nature and slightly alkaline in reaction without any problem of salinity. From the fertility point of view, it was low in available N, moderately supplied with available S, P, high in K. The results shwed that the Fe and Zn content in grain and straw were significantly influenced by various treatments of Zn and Fe. Highest Fe content in grain and straw as well as uptake by straw and grain was observed in treatment of RDF + Spraying of chelated Fe @ 2.0% (T7
). Whereas, the highest Zn content in grain and straw as well as uptake by straw and grain were observed in treatment RDF + Spraying of chelated Zn @ 1.5% (T4
). Higher nutrient uptake was observed in chelated Fe and Zn spray as compared to soil application of FeSO4
. Application of chelated Fe and Zn spray increased the efficiency of fertilizers and improved wheat nutrient content including grain Zn and Fe.