European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience

ISSN: 2321-9122

Vol. 7, Issue 2 (2019)

Detection of minimum inhibitory concentration and minimum biofilm eradication concentration among biofilm forming uropathogenic Escherichia coli for ciprofloxacin and nitrofurantoin

Author(s): Rashmi SB, Rashmi P Mahale
Abstract: The present study titled- “Detection of minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) among uropathogenic E.coli for ciprofloxacin and Nitrofurantoin” was carried out in the Dept of microbiology, J.S.S. Medical College from March to June 2016 to detect MIC and MBEC among uropathogenic E.coli. Total 150 uropathogenic E.coli isolated from patients of urinary tract infections were collected and subjected to biofilm formation among them 30 (20%) isolates showed the ability to form biofilm. Out of 150 uropathogenic isolates, 30 isolates were subjected for the detection of MIC and MBEC. Among 30 biofilm forming uropathogenic E.coli isolates, 12 (40%) isolates were sensitive and 18 (60%) isolates were resistant to ciprofloxacin in their planktonic state. For Nitrofurantoin antibiotic among 30 biofilm forming isolates, 23 (76.7%) isolates were sensitive and 7 (23.3%) isolates were intermediately sensitive in their planktonic form. Among 30 biofilm forming uropathogenic E.coli, None of the isolates showed MBEC below 16 µg/ml, 5 (16.7%) isolates showed MBEC at 16 µg/ml, 17 (56.7%) isolates showed MBEC at 32 µg/ml and rest of 8 (26.6%) isolates showed MBEC at 64 µg/ml of ciprofloxacin. For Nitrofurantoin, 23 (76.7) isolates were in the sensitive range in the planktonic state, whereas only 17 (56.7%) isolates were in the sensitive range in the biofilm state, 7 (23.3%) isolates were in the intermediate range in the planktonic state where as 8 (26.7%) isolates were in the intermediate range in the biofilm state, none were found to be in the resistant range in the planktonic state where as 5 (16.6%) isolates showed resistance to Nitrofurantoin in the biofilm state.
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