Vol. 7, Issue 2 (2019)
Biochemical characterization of bacteria responsible for bacterial black pit disease of lime (Citrus aurantifolia) and their control system
Author(s): MS Alam, S Nahar, MEK Chowdhury, MF Hasan, MA Islam, B Sikdar
Abstract: The present investigation was design to isolate and characterize the Pseudomonas syringae, bacteria responsible for citrus black pit disease and its biological control. Deviating fruits test assay in the petri dishes is more convenient and most reliable for determining the pathogenicity of Pseudomonas syringae on citrus. The bacteria isolated from infected fruits of citrus was gram-negative which showed pink color and rod shaped in gram staining procedure and the isolated bacteria was Motile. In biochemical test, Triple Sugar Iron agar test, Simmons citrate test, Methyl Red was positive. Catalase test, MacConkey agar test, Kligler Iron Agar test, Tween 80 hydrolysis test, King’s B test was negative. Tetracycline antibiotic revealed the highest activity with inhibition zone of 22.0±0.5mm diameter. In antibacterial activity assay, Allium sativum plant extract showed intermediate susceptible activity against the isolated bacteria with 12.0±0.5mm diameter zone of inhibition. Antagonistic activity of soil bacteria was found 8.0±0.5mm diameter inhibition zone against the isolated bacteria. This research would be help to identify the causal agent of the citrus black pit disease and antibiotic sensitivity assays perform its working efficiency.