Vol. 7, Issue 2 (2019)
Irradiated tumor cells lysate vaccine neighboring to silver nanoparticles attenuate immunological properties of Ehrlich carcinoma bearing mice
Author(s): Rokaya E Maarouf, Neamat H Ahmed, Amina M Medhat, El- Batal AI, Abdel Rahman B Abdel Ghaffar
Abstract: Using of irradiated tumor cells as a vaccine and metal nanoparticles may have another strategy way for cancer immunotherapy treatment. The present research examines the effect of irradiated tumor cell lysate (Lys) and/or silver nanoparticles (AgNPs) on Ehrlich carcinoma (EC). Ehrlich ascites carcinoma cells and five groups of female mice were used. Tumor size was predestined. In serum, IL-10 and IFN-γ levels were evaluated. In EC tissue CD4, CD8, caspase-3 activity, TGF-β and P53 levels and histopathological changes were examined. Mice bearing EC treated with Lys and/or AgNPs represent a significant decrease in tumor size and serum IFN-γ and IL-10 levels. In tumor tissue reduction in CD4, TGF-β and P53, increase in CD8, elevation in caspase-3 were predicted. Meanwhile, histopathological observations context with the previous results. In conclusion, Lys and/or AgNPs attenuate immunological properties of EC bearing mice.