Formulation and optimization of biodegradable insulin loaded nanoparticles
Ahmed Gamal Eid, Nazim Uddin, Samuel Girgis
This research aims to provide the diabetic patients with more adequate treatment by designing controlled release insulin loaded nanoparticles prepared by biodegradable polymers. Factors to be studied in this research are the effect of the preparation methods using two different preparation techniques microfluidic / salting out technique which is a new and promising technique and a conventional technique which is double emulsion /solvent evaporation technique. The characterization of the nanoparticles was performed in the terms of size, zeta potential, morphological analysis, IR spectrophotometry, thermal analysis, loading and In vitro release of the nanoparticles. All the formulations are showing nanoparticles in the range from 300 to 400 nm with a negative zeta potential between – 10 and – 20 mV. Furthermore, all the nanoparticles are showing smooth spherical shape with chemical and thermal compatibility between the ingredients with encapsulation efficiency that ranges from 42 % to 52 % in all formulations.