European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 7, Issue 5 (2019)

Effects of leaf and root bark of Calotropis procera and Parquetina nigrescens on protein profile of callosobruchus maculatus (coleoptera: chrysomelidae)


Alamuoye Nathaniel O

This study assessed the effects of leaf and root bark extracts of Calotropis procera and Parquetina nigrescens on the protein profile of Callosobruchus maculatus. These were with a view to developing a new biological control measure of the insect pest at molecular level. The SDS-PAGE patterns of adult male and female C. maculatus that emerged from 4th instar larvae treated with 0.5% methanolic extract of leaf and root bark of C. procera and P. nigrescens, including untreated male and female and the four instar larvae and pupa were determined. The result shows that the leaf and root bark extract of C. procera and P. nigrescens influenced changes in protein profile of C. maculatus. The extracts of C. procera leaf and P. nigrescens root bark caused an increase in the number of protein fractions of adult male and female C. maculatus as against decrease observed with the extracts of C. procera root bark and P. nigrescens leaf.
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