A comparative assessment of NaCl induced protein and antioxidant enzymes during hardening of two vegetatively propagated back mangrove species
Pradeep Kumar Maharana, Uday Chand Basak
NaCl stress application on plants including mangroves has been expanded over the last decade to know the salt tolerant behaviour. In the present study, both Excoecaria agallocha and Cerbera manghas, were vegetatively propagated and hardened against different concentration of NaCl i.e. control, 100, 200, 300, 400 and 500mM NaCl (salt acclimatization) for a period of 28days under shade-net house for the analysis of protein content and enzymatic activity. Both the species increased their protein content up to 200-300mM NaCl stress and then decreased at higher salinity. Besides this, E. agallocha leavesshowed highest protein content in comparison to C. manghas. The specific enzymatic activity of the antioxidant enzymes i.e. POX, CAT and SOD increased at higher salt stress with higher activity in C. manghas leaves for this short period of NaCl exposure and determine its sensitiveness to NaCl and may not acclimatized against long term exposure NaCl stress due to its less protein contents. Again re-establishment of salt acclimatized vegetatively propagated E. agallocha saplings can be easier than C. manghas.