The effect of carbon tetrachloride (CCl4) contaminated extract of Irvingia wombulu (kernel) on the haematological indices (PCV, Hb, WBC, RBC), serum electrolytes (Na, K+, Cl-) and serum enzymes (AST, ALT, ALP) in Albino wistar rats
Dr. Ejoba R
The toxic effect of Irvingia wombulu contaminated with carbon tetrachloride (10% CCl4) on serum electrolytes (Na, K+, Cl-), haematological indices (PCV, HB, WBC, and RBC) and serum enzymes (AST, ALT, ALP) on albino wistar rats. Twenty albino wistar rats of both sexes were divided into four groups (A, B, C, D). Group A is the control while group B, C, and Dare the test animals. The test animals (B, C and D) were fed at 100mg/Kg, 200mg/Kg and 300mg/Kg body weight of the contaminated extract for a period of twenty-one days. The animals were sacrifice and the serum electrolytes (K+, Na and Cl-), haematological indices (PCV, HB, WBC, and RBC) and serum enzymes (AST, ALP, ALT) were determined.There was significant increase in sodium, potassium and chloride ions of rats that were fed with CCl4 contaminated extract compared to the control. The serum enzymes assayed shows a decrease in ALT with an increase Alkaline phosphatase and Aspartate amino transferase as compared with the control and is dose dependent. The haematological indices shows a significance difference at P< 0.05. This indicate that the CCl4 contaminated extract is toxic to the albino wistar rats at this dose level.