Effective appraisement of hormones and Acinetobacter on salt-stress induced Cicer arietinum Cultivars
Tidke SD, Naik AA, Badhekar AK
An experiment conducted to evaluate the effect of hormones and Acinetobacter in salt-stressed induced Chickpea cultivars. In this experiment, salinity levels imposed were 4dS/m, 8dS/m and 12dS/m were tested by using conductivity meter. The stock of growth hormones was prepared as 5mg/L and 3 days incubated bacterial suspension culture (Acinetobacter spp.) was used for further study. This experiment shows that the germination percentage, seed vigor index, root, and shoot length significantly decreases with an increase in salt concentration. In the analysis of biochemical level, as the increases salt stress, there was a decrease in chlorophyll and protein content but increased in Catalase, Superoxide dismutase and proline were increased. After the treatments of Acinetobacter and growth hormones, there were different results observed in morphological and biochemical parameters. Salt stressed induced plants treated with Acinetobacter and growth hormones were showed high root-shoot length and increased in chlorophyll and protein content and decreased in Catalase, Superoxide dismutase, proline content. In SDS-PAGE analysis, both cultivars showing extra bands in increased salinity stress but after the treatment of Acinetobacter and growth hormones, these bands were disappeared. On the above result, Acinetobacter and growth hormones were responsible for maintaining the catalase, SOD, chlorophyll, proline and protein content and also responsible for regaining the root-shoot length. Hence, Acinetobacter and growth hormones treated plants show a better result to overcome problems that occurred with salt stress. Growth hormones showed a better result than Acinetobacter, Specially NAA worked more prominently than IBA. NAA shows better results as compared to IBA on chickpea and nullifies the effect of salt stress and overcome the problem occurred with salt stress.