A review on types of polymerase chain reaction for diagnosis and cloning technique
Anushya C, Soumiya PR, Swetha K, Prabhu N
PCR is a simple, ingenious, enzymatic assay, which allows specific DNA fragment to amplify from a complex pool of DNA. In earlier days for the first it allowed to detect specific region and large amounts of DNA from this method. These strategies in PCR have propelled huge scientific endeavors such as the Human Genome Project (HGP). The technique is currently widely used by clinicians and researchers for disease diagnose, clone and sequence genes, and carry out refined quantitative and genomic studies in a hasty and very receptive manner. PCR technique is not only used for diagnosing disease but also used in identifying inhibitor or enhancers in food samples. It is also used in the detection of Apolipoprotein E Genotype by DNA electrochemical biosensor coupled with PCR. This article describes the process involved in PCR and its types which is majorly used in diagnose and cloning technique.