Study of the antimicrobial activity of consortiums of plant extracts and actinomycetes against multi-drug resistant bacteria
Dhiraj Chaudhari, Rupali Gaikwad, Dr Bela Nabar
In order to ease the burn wound healing the bacterial accumulation has to be alleviated.It successively upsurges the necessity to improve substitute approach to counter the growing apprehension against the multi-drug resistant infection in burn wounds. In the present study, two combinations of herbal alcoholic extracts have been assessed for antimicrobial activity against the isolates collected from human burn wound sample and biopsies. Total of 42 strains have been isolated and screened for its antibiotic resistance. It was found that a significant amount of bacteria adapted resistance to traditional antibiotics such as Gentamycin and Imipenem. Out of the 42 isolates, 28 isolates were found to be multi-drug resistant strains. The study demonstrated the inhibition of resistant strain of Vancomycin-resistant Staphylococcusaureus (VRSA) by extract of Terminalia chebula fruit, Terminalia bellirica fruit, Syzygium aromaticum fruit, Syzygium cuminifruit (HBLJ) while multi-drug resistant Escherichia was inhibited by consortium of Ocimum tenuiflorum Leaves, Ficus religiosa Bark and Piper betel Leaves (TPB) extract. Chloramphenicol, Kanamycin and Tetracycline effectively inhibited All 7 gram positive bacteria. Significantly, actinomycetes showed greater antimicrobial activity, which has inhibited the great number of multidrug resistant bacteria. Kill time study indicated that HBLJ extract inhibited Pseudomonas spp inhibited within 1 hour of contact time. But TPB extract showed relatively high contact time for efficient bioactivity. Moreover, both of extract showed no anticancer activity in 10µg/ml to 80µg/ml in case of SK-MEL-2 cell line, indicates extract selectively inhibited bacteria.