The fruit and vegetable waste i.e. peels, pomace and seeds can be utilized to produce various products such as ethanol, single cell protein, hydrogen. Single Cell Protein (SCP) is one of the product that can be isolated by use of fruit and vegetable waste. SCP refers to dried microorganisms that can be used as protein dietary supplement. The production of SCP majorly depends upon the type of substrate being provided to the microorganism. Studies have shown that peels have high nutritional value and hence can be used to produce a value added product. Comparison was made in the single cell protein production by using different fruit and vegetable wastes. Saccharomyces cerevisiaewas allowed to grow on different substrates like peels of pomegranate, orange, carrot and banana to produce SCP. The comparative study showed that carrot waste produced maximum amount of Single Cell Protein.