In vitro culture establishment and seed studies of Diospyros buxifolia (Blume) hiern: A slow growing timber species of Western Ghats
Tresa Hamalton, Rekha Jaganathan, Almas Khanam
Diospyros buxifolia is a timber species, conventionally propagated through seeds, which have very short viability. A protocol for in vitro culture establishment of D. buxifolia using leaves, nodes and seeds as explants is reported. In this study, morphological characters of the fruits and seeds were also examined. Surface sterilization of explant is the first and most important step in establishing in vitro culture. Under the regime employed in this study, the most critical factor was the use of autoclaved antioxidant solution. For in vitro culture establishment, explants (leaf and node) collected in autoclaved antioxidant solution, followed by pretreatment with high concentration of M-45, Streptomycin and HgCl2 significantly increased number of aseptic cultures (>95%). The method reported herein for in vitro establishment of D. buxifolia cultures can be successfully used for conservation strategies of D. buxifolia which is otherwise a slow growing tree.