Gelling behaviour of alternative biogel in tissue culture
Bello OA, Abiola AB, Awodeji WA
Guinea corn (G.C) Shorghum bicolor (L.) Moench] and Semovita (S.V) Triticum turgidum (powder) were investigated as agar substitutes. The medium supplemented with 0.2mg/L α-naphthalene acetic acid (NAA) and 0.5mg/L 6-benzylaminopurine (BAP). G.C flour (white variety) and S.V were added at 0-100 g/L (0:1 to 2:1). Medium with either G.C or S.V flour, thick slurries of the starch were made by continuously heating the medium at a temperature of 95 ± 5oC, with vigorous stirring at intervals for 10 - 15 min and allow to cool to study their gelling behavior by turning the vessel after 24 h. Media gelled with 60, 80 and 100g/l of guinea corn and semovita with combination of agar (0:1 to 2:1) formed solid media. Media gelled with G.C and S.V alone at 60g/l formed a runny matrix while G.C and S.V (80 and100 g/l) at 1:0 and G.C (100 g/l) with combination of agar (2:1) formed semi-solid media. Media gelled with 60, 80 and 100g/l of G.C and S.V (0:1 to 2:1) were found to be promising alternate candidate for agar.