European Journal of Biotechnology and Bioscience

Authors
Anannya Mohapatra, V. Madhava Rao, Dr. Mala Ranjan

Abstract
Crude Bromelain was extracted from peel, pulp & stem of the pineapple by using sodium acetate buffer. Then those were purified separately by ammonium sulphate precipitation, Dialysis followed by ion-exchange chromatography. Enzyme Activity & Specific activity of peel, pulp & stem bromelain were estimated and protein fold and total enzyme yield were calculated. Enzyme kinetics was studied for bromelain by taking effect of temperature, pH, Substrate Concentration, Activator & Inhibitor. Finally peel part gave the better yield of enzyme & the specific activity was more than the pulp & stem.