European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 1, Issue 2 (2013)

Comparative study of the increased production & biochemical characterization of Bromelain from the waste & edible parts of pineapple (Anannus commas)


Anannya Mohapatra, V. Madhava Rao, Dr. Mala Ranjan

Crude Bromelain was extracted from peel, pulp & stem of the pineapple by using sodium acetate buffer. Then those were purified separately by ammonium sulphate precipitation, Dialysis followed by ion-exchange chromatography. Enzyme Activity & Specific activity of peel, pulp & stem bromelain were estimated and protein fold and total enzyme yield were calculated. Enzyme kinetics was studied for bromelain by taking effect of temperature, pH, Substrate Concentration, Activator & Inhibitor. Finally peel part gave the better yield of enzyme & the specific activity was more than the pulp & stem.
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