Molecular characterization of protease producing Bacillus sp. isolated from kitchen soil using RAPD markers
Amongst the industrial enzymes, proteases hold a major significance and are used in various industries like soaps and detergents, brewing, meat processing, leather, dairy, pharmaceutical and food. Proteases are one of the many extra-cellular enzymes that are produced by Bacillus sp., which are a group of industrially important microorganisms. In the present study 22 isolates were obtained from the kitchen soil samples. These isolates were then screened for their protease-activity through the plate assay using skimmed Milk Agar. Five isolates showing high protease-activity were selected, which were further characterized based on their colony characteristics, morphological and biochemical characteristics. The genetic variability of these protease producing Bacillus sp. were analysed using RAPD-PCR technique. In the bands obtained using primer A11, 92.30% were polymorphic and only 7.69 % were monomorphic and with C9, 92.7% were polymorphic and only 7.27 % were monomorphic. This method of molecular characterization using random primers implements a rapid identification and the diversity of the Bacillus sp.
Prasad. M.P.. Molecular characterization of protease producing Bacillus sp. isolated from kitchen soil using RAPD markers. European Journal of Biotechnology and Bioscience, Volume 2, Issue 3, 2014, Pages 38-42