Molecular characterization of Van a gene from vancomycin resistant Staphylococcus aureus isolated from clinical samples
Vancomycin is commonly used for patients with Staphylococcus aureus infections, and reduced susceptibility to vancomycin is becoming increasingly common. Therefore the treatment of Staphylococcus aureus infections is becoming increasingly more complicated due to the emergence of various types of antibiotic resistance. The resistance in these organisms is conferred by several vancomycin resistance genes (Van) like VanA, VanB, VanD, VanC and VanM. Knowledge of presence of Van genes in various organisms was felt necessary prior to molecular investigation. The present study was aimed at focusing on the prevalence of vancomycin intermediate and vancomycin resistant Staphylococcus aureus (VISA and VRSA respectively) by amplifying the presence of Van-A gene isolated from various clinical samples from Bangalore. Results indicated that out of 18 Staphylococcus aureus strains isolated from clinical samples, 5strains of Staphylococcus aureus showed presence of Van-A gene after amplification. The present findings provides the data about emergence of vancomycin resistance Staphylococcus aureus which instigates the scientists to further investigate on the significance of these genes and emphasis is placed on using molecular and bioinformatics tools to help minimize acquired resistance in antibiotics.
Prasad. M.P.. Molecular characterization of Van a gene from vancomycin resistant Staphylococcus aureus isolated from clinical samples. European Journal of Biotechnology and Bioscience, Volume 2, Issue 3, 2014, Pages 43-47