European Journal of Biotechnology and Bioscience

Authors
Rashmi KS, Santosh Mayannavar, Keerthi Deshpande, Ganaraja B

Abstract
Aims and objectives: To elucidate role of Orexin B in the control of ingestive behaviour mediated through nucleus accumbens. Materials and Methods: In the present study Orexin B was infused into the nucleus accumbens in Wistar albino rats. Fifty four male Wistar rats, divided into three groups of 18 each, were further divided into three subgroups as Control, Sham operated control and experimental group (n=6 each). In the experimental group, Orexin B was administered bilaterally into the Nucleus accumbens in overnight fasted animals, at a dosage of 3nmol/µl (group 1) and 30nmol/µl (group 2) and their food intake and water intake were measured meticulously after 1 hour, 2 hours, 4hours, 6 hours,12 hours and 24 hours. Further to establish the role of Orexin B unequivocally, OX2R antagonist TCS-OX2-29 was infused into NAcc bilaterally in the third group and their feeding behaviour was assessed. Results: In low doses of Orexin B (3nm/µl) there was slight increase in food and water intake. But difference was not statistically significant, except increase in water intake at the end of first hour (p<0.05). The high dose of Orexin B (30nm/µl) increased food and water intake significantly at the end of 1, 2, 4, 6 hour, 12 hour and 24 hours, compared to control and sham operated rats. The infusion of the Orexin B antagonist (TCS-OX2-29) resulted in decreased food and water intake significantly at the end of 2, 4, 6, 12 hour and 24 hours.