European Journal of Biotechnology and Bioscience

Authors
Bidisha Mondal, Sankar Pramanick, Ramkrishna. Saha

Abstract
Citrus aurantifolia is an important fruit crop of Eastern India. The region is one of the centre of origin of the crop and harbours excellent genotypes. For genotypic documentation of the resource 12 representative plant types of West Bengal were selected for molecular analysis using 28 decamer primers. Out of which 14 primers proved useful generating 64 amplicons of which 58 were polymorphic. Primer OPB 02 and OPAT 04 very effective with highest PIC. A Dendrogram was constructed using Squared Euclidean Distance using Ward’s method which reflected no apparent relation between genetic diversity and geographical distance. Some distant genotypes grouped together while genotypes from a single location fall on different clusters. 9 primers namely OPAT04, OPB10, OPAA10, OPAD10, OPM05, OPB02, OPH11, P140 and P141 were useful generating unique amplicons marking specific genotypes. The study revealed the occurrence of high level of genetic diversity and proved the efficiency of RAPD markers for exploring the same.