European Journal of Biotechnology and Bioscience

Authors
N’dodo boni clovis koffi, Marie Noël Yeyeh Toualy, Kouamé Daniel Kra, Hortense Atta Diallo

Abstract
In vitro and in vivo tests were conducted to evaluate P. fluorescensantagonistic properties against P. aphanidermatum. Four discs of 5 mm diameter taken from P. fluorescens cultures were placed on PDA media, 3 cm a part from central disc (10 mm diameter) covered with fungus. Antagonism was verified by observing the presence (+) or absence (-) of an inhibition zone between the antagonist and the pathogen using a light microscope and on culture media. For the in vivo experiments, four treatments were made: plants treated + bacteria + pathogen, untreated + bacteria + pathogen, plants inoculated with the pathogen and healthy control. In vitro test showed antifungal activities of P. fluorescens against P. aphanidermatum. Mycelia in the inhibition zone were altered completely when observed under the microscope and could not grow on new culture media. Application of bacteria reduced disease incidence to 18 %.