The In-Vitro Effect of Aqueous Leaf Extract of Basella Alba L. On Osmotic Fragility of Red Blood Cells in Hbss Subjects
Arokoyo Ds, Seriki Al, Babatunde Ld, Ojo Ao, Bamidele O
The aqueous leaf extracts of Basella alba were investigated in the present study for their in-vitro effect on red blood cell fragility inrnclinically confirmed HbSS subjects. A total of 12 human subjects, six (6) male and six (6) female with HbSS were used for thisrnstudy. In-vitro osmotic fragility was determined spectrophotometrically by obtaining optical density at wavelength of 540nm. 5-6 mlrnof blood was obtained from each subject and analyzed with the use of 15 centrifuge tubes, divided into 5 per group. The first grouprnserved as the control (without Basella alba), the second group contained low dose (0.2mg/ml) of Basella alba and the third grouprncontained high dose (0.4mg/ml) of Basella alba in varying saline percentages of 0.0%, 0.3%, 0.6%, 0.9% and 1.2%. Hemolysisrnoccurred at all saline percentages; however, low dose and high dose of aqueous extract of Basella alba reduced the percentage ofrnhemolysis in all subjects, especially in the males. This suggests that there might be a possible synergistic activity between Basellarnalba and androgens, by which Basella alba reduces hemolysis of red blood cells, combined with its anti-anemic effect, thereby,rnmaintaining the high red blood cell count seen in males. Thus, it is advised that Basella alba should be consumed regularly byrnsicklers, in order to help reduce the occurrence of hemolytic anemia.
Arokoyo Ds, Seriki Al, Babatunde Ld, Ojo Ao, Bamidele O. The In-Vitro Effect of Aqueous Leaf Extract of Basella Alba L. On Osmotic Fragility of Red Blood Cells in Hbss Subjects. European Journal of Biotechnology and Bioscience, Volume 3, Issue 10, 2015, Pages 05-10