European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 3, Issue 11 (2015)

Evaluation of different botanical extracts on the linear colony growth of the fungus Fussarium wilt of mango nursery and its in-vitro control


Abdul Salam Mengal, Shahid Hussain, Manzoor Ali, Abro, Abd-Ur-Rehman Khalid Sajjad Ahmed Maari, Ghullam Hussain Jatoi, Tahira Nisa, Muhammad Rafiq, Sohail Iqbal

Fusarium wilt is one of the most serious and destructive diseases of mango nursery throughout Pakistan. This disease cause hugernlosses in mango nursery upto 22-25%. Keeping in view the losses caused by the Fusarium wilt in mango nursery, the presentrnstudies were planned to see the present position of the problem of Fusarium wilt disease by survey of disease incidence in differentrndistricts and to manage the disease by various methods of management like, use of chemicals and botanical extracts. The mainrnobjectives of the study were, (i) to record the disease incidence of Fusarium wilt in different mango nurseries of two districts ofrnSindh including, Hyderabad and Mirpurkhas (ii) to evaluate certain botanical extracts to manage this disease. Whereas, botanicalrnwere selected such as neem, Akk, Dhatura, Tomak and Tooh and the concentrations of botanical extracts were kept as (1, 2, 3 ml).rnAll the experiments were carried out according to the Randomized Completely Block Design (RCBD) with five treatments andrnthree replications.
The results of our study showed that the disease is present in high frequency throughout all the districts surveyed. The maximumrndisease incidence of Fusarium wilt was recorded from Mirpurkhas (60.0%) followed by Hyderabad (55.0%). All the used botanicalrnextracts showed high variation in reducing the linear colony growth of the fungus.The botanical extracts significantly reduced thernlinear colony growth of the fungus, Fusarium oxysporum. Among all of them, the most effective botanical extract was Neemrnwhich reduced the linear colony growth of the fungus up to (24.17 mm) followed by, Akk (31.17 mm). Whereas, the Datura reducernthe linear growth of the fungus up to (34.33 mm) while, the other two botanical extracts Tomak and Tooh were found less effectivern(43.83) againstFusarium oxysporum as compared to control (86.83). So, we can conclude that for the management of Fusariumrnwilt of mango nursery the more suitable through plant extracts. Whereas, Neem and Akk can also be used effectively as anrnalternate to chemical fungicides and can manage the disease as well. These studies will be proved fruitful for researchers andrngrowers in future for management of the Fusarium wilt of mango nursery.
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How to cite this article:
Abdul Salam Mengal, Shahid Hussain, Manzoor Ali, Abro, Abd-Ur-Rehman Khalid Sajjad Ahmed Maari, Ghullam Hussain Jatoi, Tahira Nisa, Muhammad Rafiq, Sohail Iqbal. Evaluation of different botanical extracts on the linear colony growth of the fungus Fussarium wilt of mango nursery and its in-vitro control. European Journal of Biotechnology and Bioscience, Volume 3, Issue 11, 2015, Pages 07-14
European Journal of Biotechnology and Bioscience