European Journal of Biotechnology and Bioscience

Authors
Randa S Hana, Mohamed M Ayyad, Heba A Yassa

Abstract
Objectives: Study the effect of hyperleptinaemia (systemically and locally) and hyperglyceamia on Fibroblast-specific protein-1rngene expression, atrial natriuretic peptide (ANP) level, and extent of calcification in stenotic aortic valves, the role of FSP1 as arnmarker of valve calcification, the role of aortic valve repair in ameliorating the metabolic alterations and study the sensitivity, rnspecificity of ANP, leptin and NO as diagnostic and prognostic markers in valve diseases.Design and Methods: FSP-1(by qRT-PCR and western blotting), atrial natriuretic peptide, glucose, insulin and leptin (by ELISA), rnnitric oxide and calcium (colorimetrically) were studied in 20 aortic stenosis patients and 10 controls.Results: In valve tissues, high leptin was significantly associated with increased FSP1 and calcium deposition, 66% of patients hadrnglucose intolerance with increase in the studied parameters and decreased to 36% after surgery.Conclusion: FSP-1 is a useful marker for valve calcification. Valve surgery, by decreasing leptin, ANP and NO had a beneficial rolernin improving insulin resistance, Elevated leptin and ANP levels, not insulin resistance, were associated with poor survival.