European Journal of Biotechnology and Bioscience

Authors
Erkings Michael Y, Dharmar K

Abstract
Eugenia singampattiana Beddome belongs to the family Myrtaceae. It is an evergreen medicinal tree found at the tail end of SouthernrnWestern Ghats of India. It is a critically endangered species enlisted by IUCN and exists only in with two fragmented population. Itrnwas successfully propagated through in vitro culture on MS medium. BAP are most suitable than Kn that represent the 3.66±0.21rnshoot number and 3.75±007 shoot elongation but shoot regeneration percentage more on Kn in 1.5 mg/l concentration (53%). 2.0rnmg/l of 2, 4-D alone and 2.5 mg/l 2,4iP alone and combination of 2,4iP (2.0 mg/l)+Kn (0.5 mg/l) and 2,4iP (2.5 mg/l)+Kn (0.5 mg/l)rnare intense callus formation. The in vitro callus generation, shoot regeneration and root formation are prerequisite for the massrnmultiplication which is liable part of strategies for ex situ conservation.