European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 3, Issue 2 (2015)

Determination of genetic diversity among antibiotic resistant E. coli strains using RAPD molecular markers


Hazel Leena George, Prasad M.P

Urinary tract infection (UTI) is a common problem in pregnant woman. Escherichia coli are known as common microorganism that exists in the intestine tract of human and warm-blooded animals. In the present investigation E.coli were isolated on EMB agar from urinary tract infected of pregnant women from hospitals in and around Bangalore. A total of 352 E.coli samples were obtained from different patients. Of these 29 samples were found to be resistant to multiple drugs and were used for further investigation for determining phylogenetic Analysis. The DNA isolated was used for RAPD analysis using 10 different primers namely OPV 12, OPA 13, OPA 08, OPR 08, OPU 17, OPA 11, OPU 14, OPD 19, OPS 03, OPA 10 and OPD 20. The RAPD of the samples with the primers showed multiple banding patterns with bands ranging from 5 to 13 bands per sample. The average bands were found to be 8 bands. The band size varied from 100bp - 1500 bp when compared with standard 500bp ladder. The phylogenetic analysis showed that the isolates were mainly of 5 different sub types which varied to each other to a smaller extent.
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How to cite this article:
Hazel Leena George, Prasad M.P. Determination of genetic diversity among antibiotic resistant E. coli strains using RAPD molecular markers. European Journal of Biotechnology and Bioscience, Volume 3, Issue 2, 2015, Pages 20-25
European Journal of Biotechnology and Bioscience