Effects of Ethylacetate and n-Butanol Fractions of Acacia nilotica Extract on Some Haematological Profile and Serum Electrolytes Levels of Alloxan-induced Diabetic Wistar Rats
Y. Tanko, M.F.Obafemi, A. Muhammad, E.D. Eze, A. Jimoh, K.A. Mohammed, M.A.Dewu, A. Mohammed
The aim of this study was to examine the effects of ethylacetate and n-butanol fraction of Acacia nilotica fractions on some haematological parameters and serum electrolytes levels of alloxan-induced diabetic wistar rats. Thirty (30) Wistar rats were used in this study. Group 1, administered distilled water, served as negative control; Group 2: administered insulin 6.iu/kg, served as positive control; Group 3: administered 50mg/kg b.w of ethyl acetate fraction; Group 4: administered 100mg/kg b.w of ethyl acetate fraction; Group 5: administered 100mg/kg b.w of n-butanol fraction and Group 6: Administered 200mg/kg body weight of n-butanol fraction. All regiments were given intraperitoneally once daily to the animals for a period of two weeks .At the end of the two weeks, the animals were sacrificed and the blood samples were collected for biochemical assays. Theresults obtained showed that there was no statistically significant change (P>0.05) in the packed cell volume, red cell count, haemoglobin concentration and as well as white blood cells and its differential counts in groups treated with all doses of ethyl acetate and n-butanol fractions ofAcacia nilotica in the experimental animals when compared with the diabetic control untreated group. There was significantly increase (p<0.05) urea level, potassium and chloride ions concentrations in the group treated with 100 mg/kg b w ethyl acetate. As regards to the 200 mg/kg of the n-butanol fraction there was a significant increase in the level of urea when compared with the control untreated group.
Y. Tanko, M.F.Obafemi, A. Muhammad, E.D. Eze, A. Jimoh, K.A. Mohammed, M.A.Dewu, A. Mohammed. Effects of Ethylacetate and n-Butanol Fractions of Acacia nilotica Extract on Some Haematological Profile and Serum Electrolytes Levels of Alloxan-induced Diabetic Wistar Rats. European Journal of Biotechnology and Bioscience, Volume 3, Issue 3, 2015, Pages 31-34