European Journal of Biotechnology and Bioscience

Authors
Rahul Shivaji Patil, Komal Kundan Bhise

Abstract
The limited data on the toxicity of medicinal plants leads us to continue researches to increase and / or improve the results available, in order to better appreciate the therapeutic doses to use. This study aimed at determining the biochemical and hematological profiles during the acute oral toxicity of ethanol extract of Gomphrena celosioides in rats. The acute oral toxicity was evaluated in rats according to the guideline n⁰ 423 of the OECD. Under this method, the starting dose is selected from these four, 5, 50, 300 and 2000 mg / kg. The level chosen is the one for which we can expect to see mortality among treated animals. The absence of treatment-related mortality at the initial dose determines the next step. The animals were daily observed for 14 days after treatment. On the fifteenth day, the animals were killed and dissected. Their blood and organs (heart, kidneys and liver) were collected for biochemical and hematological analyzes. The results showed that the LD50 by oral route in rats was greater than 5000 mg / kg body weight. The relative weights of organs (kidneys, liver and heart) and hematological parameters were not significantly different (P> 0.05). Biochemical analyzes showed a significant increase in transaminases ALT and AST (P˂0.05) at a dose of 5000 mg / kg. This study suggests that the indiscriminate use of the ethanol extract of Gomphrena celosioides should be avoided because with the dose of 5000mg / kg, it would induce hepatic dysfunction.