Virulence of five soil isolates (WRMa1, WRMa3, WRMa7, MTCC6060 and MTCC 6067) from the Tamil Nadu, India region and two commercial isolates (designated as MMa4, MMa5) of Metarhizium anisopliae was tested against third instar larvae and adult female of Aedes aegypti and Culex quinquefasciatus. The WRMa3 produced significantly higher mortality than other isolates against third instars, with a lethal threshold level (LT50) of 4.2 (±0.16) days for Ae. aegypti and 3.9 (±0.18) days for C. quinquefasciatus respectively. The same isolate produced 75% mortality, with LT50 of 5.61 days and LC50 of 2×104 conidia/ml in adult female Ae. aegypti. With C. quinquefasciatus, 96% mortality was observed with an LT50 of 3.2 days and an LC50 of 1.8×108 conidia/ml. The effective isolate, WRMa3, was tested further against the survival of the both mosquitoes at higher dose of 2×108 conidia/ml. The total life span of fungus-treated mosquitoes of Ae. aegypti and C. quinquefasciatus were significantly reduced when compared to uninfected mosquitoes. The results indicate that the WRMa3 soil isolate of M. anisopliae can be effective in the control of C. quinquefasciatus and Ae. Aegypti larvae and adults.
Kirubakaran.S.A, Haripriya.R, Naveenraj.D, P.Thirumalaivasan. Virulence of Metarhizium anisopliae soil and commercial isolates against Culex quinquefasciatus Say, a vector of Bancroftian filariasis, and Aedes aegypti L, a vector of dengue fever. European Journal of Biotechnology and Bioscience, Volume 3, Issue 3, 2015, Pages 39-47