European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 3, Issue 5 (2015)

Frequency and susceptibility pattern of Multidrug Resistant Pseudomonas aeruginosa in isolates of patients from a tertiary care hospital of Karachi, Pakistan


Kanaan Mansoor, Syed Bilal Tanvir, Ali Shariq, Arif Hussain, Badar Jahan Farooqi, Sumera Ahmed, Roofia Tanvir

Objective: To determine the frequency and susceptibility pattern of multidrug resistant P.aeruginosa isolates from clinical specimens in a tertiary care hospital in Karachi, Pakistan. Methods: A cross sectional study was performed over a time frame of 2 years, from January 2013 to January 2015 at a tertiary care private hospital in Karachi. These specimens were collected from three different tertiary care units situated at three different locations of the city. Standard and specific microbiological methods were used to identify the clinical isolates. The isolates were cultured on chocolate and MacConkey agar. The susceptibility patterns were deduced by utilizing Kirby Bauer Disc diffusion method on Mueller-Hinton Agar. Results: A total of 634 isolates of P. aeruginosa were cultured during this study’s time frame ranging from January 2013 to January 2015. Positive cultures were then tested against 9 antibiotics from the
following classes of drugs: B-Lactams, Carbapenems, Aminoglycosides and Fluoroquinolones. 36.5% of the samples were found to be resistant to 3 or more antibiotics, were labeled as MDR P. aeruginosa. The most sensitive drug was Colistin (100%), followed by Ciprofloxacin (66.8%), Piperacillin/Tazobactum (49.1%), Cefaperazone/Sulbactum (47.4%) and Cefepime (44%). Conclusions: MDR P. aeruginosa isolates show a progressive trend as compared to previous studies. The increased resistance pattern of isolates to multiple drugs was noteworthy.
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How to cite this article:
Kanaan Mansoor, Syed Bilal Tanvir, Ali Shariq, Arif Hussain, Badar Jahan Farooqi, Sumera Ahmed, Roofia Tanvir. Frequency and susceptibility pattern of Multidrug Resistant Pseudomonas aeruginosa in isolates of patients from a tertiary care hospital of Karachi, Pakistan. European Journal of Biotechnology and Bioscience, Volume 3, Issue 5, 2015, Pages 33-36
European Journal of Biotechnology and Bioscience