European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 3, Issue 5 (2015)

Keratinolytic potential of fungi isolated from soil preserved at the Micoteca URM


Minelli Sousa, Odacy Souza, Marília Maciel, Roberta Cruz, Maria Gorete Rêgo, Oliane Magalhães, Adalberto Pessoa-Júnior, Ana Porto, Cristina Souza-Motta

Keratinous materials are insoluble and resistant to degradation by common proteolytic enzymes; it is therefore important to study the microorganism producers of such enzymes for use in the biotechnology industry. Fifty isolates of fungi were checked to determine if they had the ability to use keratin as a substrate. They were grown in a submerged culture in a medium containing poultry feathers and salts. Aspergillus sulphureus URM 5029, Trichoderma aureoviride URM 5574, A. avenaceus URM 5051 and A. sclerotiorum URM 5586 gave the best results with 7.35 U/ml, 7.2 U/ml, 6.7 U/ml and 6.05 U/ml, respectively, of keratinolytic activity. The keratinase from A. sulphureus URM 5029 showed better activity at pH 10.0 and 35 ºC. The stability of keratinase from A. sulphureus URM 5029 over wide ranges of pH (6.5–9.0) and temperature (25–60 °C) suggests the feasibility of using this strain in commercial biotechnological processes
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How to cite this article:
Minelli Sousa, Odacy Souza, Marília Maciel, Roberta Cruz, Maria Gorete Rêgo, Oliane Magalhães, Adalberto Pessoa-Júnior, Ana Porto, Cristina Souza-Motta. Keratinolytic potential of fungi isolated from soil preserved at the Micoteca URM. European Journal of Biotechnology and Bioscience, Volume 3, Issue 5, 2015, Pages 10-15
European Journal of Biotechnology and Bioscience