European Journal of Biotechnology and Bioscience

European Journal of Biotechnology and Bioscience


European Journal of Biotechnology and Bioscience
European Journal of Biotechnology and Bioscience
Vol. 3, Issue 5 (2015)

In vitro seed germination, somatic embryogenesis and protocorm based micro propagation of a terrestrial ornamental orchid - spathoglottis plicata blume


Y.Ruby Stella, T.Amutha Priya,K. Mydeen Fathima Begam, V. Manimekalai

Spathoglottis plicata Blume, is a beautiful terrestrial orchid species with long grassy leaves. Orchids are commercially important flowers and potted plants with in floriculture trade are valued word wide. It is considered outstanding in the ornamental flowers trade due to its diverse colors, shapes, forms and long lasting blooms. In this investigation an attempt was made to propagate the plant by tissue culture techniques. In vitro regeneration potential of seeds was investigated. These seeds were cultured on Murashige and Skoog’s Medium with (or) without hormones. 6 Benzal Amino Purine (BAP) and Kinetin at 10µM concentration was effective in inducing callus on seed explants. Further, periodical subculture and maintenance resulted in the development of somatic embryos which subsequently formed Protocorm like Bodies (PLBs). PLBs developed into complete plantlets forming micro shoots and roots. On an average six micro shoots were formed from every PLB. The developed plantlets were transferred to polycups containing artificial soil – vermiculite. Synthetic seed technology is today an important tool for the in vitro propagation and conservation of ornamental plants. PLBs formed in vitro were encapsulated in 3% sodium alginate matrix to form synseeds. Encapsulation ensures slow growth and long term preservation. These seeds are being maintained in cold storage and could be used in future for germination to produce new plantlets. The protocol developed in the present investigation can be commercially exploited and used as a means for the rapid regeneration of this terrestrial orchid – Spathoglottis plicata.
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How to cite this article:
Y.Ruby Stella, T.Amutha Priya,K. Mydeen Fathima Begam, V. Manimekalai. In vitro seed germination, somatic embryogenesis and protocorm based micro propagation of a terrestrial ornamental orchid - spathoglottis plicata blume. European Journal of Biotechnology and Bioscience, Volume 3, Issue 5, 2015, Pages 20-23
European Journal of Biotechnology and Bioscience