Large scale production of Ruta graveolens. L shoots using aerated bioreactor
Ajit A. Kengar, Govind T. Paratkar
Ruta graveolens is important medicinal and aromatic plant of pharmaceutical value. To meet thernindustrial requirements and optimize production and decrease cost of seedlings, the bioreactor helps lot.rnThe main aim of this work was to compare in vitro cultivation of Ruta graveolens. L different systems ofrnliquid culture with simple aerated bioreactors. The in vitro growth of the shoots was promoted inrntraditional system and in aerated bioreactor. Both propagation systems used the basic MS liquid mediumrnsupplemented with 4.44 µM BA+2.84 µM IAA. The cultures were kept into culture room for 30 daysrnwith controlled temperature at 25 ±2 0C under white cold light (3000 lux) with photoperiod of 16 hours.rnAnd evaluation was done. Among the two systems, bioreactor system showed the best results for shootsrnand biomass production compared with the traditional system. So the aerated bioreactor system turns intorneffective technique to produce shoots of R. graveolens in large scale.